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cdaR [2019-08-26 09:20:49]
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cdaR [2019-08-26 09:20:49]

effector protein controlling [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|CdaA] diadenylate cyclase activity
locus
BSU_01760
pI
4.72
mw
52.78 kDa
protein length
483 aa Sequence Blast
gene length
1452 bp Sequence Blast
function
regulation of c-di-AMP synthesis
product
effector protein controlling [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|CdaA] diadenylate cyclase activity
essential
no
synonyms
ybbR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]

    • Gene

    Coordinates
    197,027 → 198,478

    The protein

    Catalyzed reaction/ biological activity

  • stimulates [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|CdaA] activity upon protein-protein interaction [Pubmed|23192352]

    • Structure

  • [PDB|2KPU] (one YbbR domain, from ''Desulfitobacterium hafniense'') [Pubmed|21154411]

    • [SW|Localization]

  • cell membrane, oriented towards the outside [Pubmed|26240071]

    • Expression and Regulation

    Operons

    genes
    [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]
    description
    [Pubmed|23192352]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22211522], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]

    • Biological materials

    Mutant

  • GP999 (''[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP985 (''[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]''-''[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • BKE01760 (Δ[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCCCAGCGGTTGTTTA, downstream forward: _UP4_GAATAAAAAAGGAGCGATTA
  • BKK01760 (Δ[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGCCCAGCGGTTGTTTA, downstream forward: _UP4_GAATAAAAAAGGAGCGATTA

    • Expression vectors

  • IPTG inducible expression of Strep-''cdaR'' in ''E. coli'': pGP2565 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • IPTG inducible expression of His-''cdaR'' in ''E. coli'': pGP2557 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab
  • GP2031: ''cdaR-Strep aphA3'', chromosomal expression in B. subtilis, for [protein|search|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab. Respective plasmids: pGP1989 and pGP1992.

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 25869574,30224435

    • Original publications

  • 12884008,16236721,26240071,23192352,22211522,21154411,26025048,26527648,27834680